April 2008, Sunnyvale, CA—Dionex announces a new method that simplifies determination of active ingredients in ophthalmic solutions. Application Note 194: Determination of Carbachol in Ophthalmic Solutions Using a Reagent-Free™ Ion Chromatography System describes a streamlined procedure for quantifying carbachol, choline, and bethanacol chloride in these solutions. The method uses suppressed conductivity in conjunction with a Reagent-Free™ ion chromatography (RFIC™) system. In contrast, the current USP monograph (USP 30-NF 25) uses colorimetric detection, which is labor-intensive and can be subject to interference from other analytes. The method described in Application Note 194 provides baseline resolution of carbachol, bethanecol, and choline in the solution, with minimal sample preparation and no time spent preparing mobile phase or postcolumn color reagents.
Carbachol is used to lower interocular pressure in patients with glaucoma, and as a rinse solution in ophthalmic surgery. The method described in this Application Note allows reliable quantitation of carbachol with a linear range from 0.02 mg/L to 1000 mg/L.
Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and extraction systems, consumables, and software for chemical analysis. The Company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.
Reagent-Free and RFIC are trademarks of Dionex Corporation.
