September 2008, Sunnyvale, CA—Dionex announces a new method for determining the amount of Mannose-6-phosphate (M-6-P) in a glycoprotein. Application Note 202: High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Analysis of Mannose-6-Phosphate describes the use of an ICS-3000 ion chromatography system, CarboPac® analytical columns, and electrochemical detection to both determine the amount of mannose-6-phosphate (M-6-P)in a glycoprotein and confirm its presence.
D-Mannose-6-phosphate is a terminal monosaccharide of asparagine-linked oligosaccharides. It is an important intermediate in glycoprotein production, and deficiencies in M-6-P can lead to numerous congenital disorders. M-6-P-containing proteins are an active area of medical research, with the goals of understanding and hopefully treating such disorders. The new method described in this application note fully resolves mannose-1-phosphate (M-1-P) and M-6-P, and M-6-P from the protein acid hydrolysis products created during the release of M-6-P from the glycoprotein.
Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and extraction systems, consumables, and software for chemical analysis. The Company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.
CarboPac is a registered trademark of Dionex Corporation.
