Proswift Reverse Phase Specifications

Reversed-Phase
Column name	PepSwift ProSwift RP-1S ProSwift RP-2H ProSwift RP-3U
Column dimensions (i.d. x length)	100 µm x 5 cm 200 µm x 5 cm 500 µm x 5 cm 4.6 × 50 mm 4.6 × 50 mm 4.6 × 50 mm
Base matrix material	Polystyrene-divinylbenzene Polystyrene-divinylbenzene Polystyrene-divinylbenzene Polystyrene-divinylbenzene
Surface chemistry	Phenyl Phenyl Phenyl Phenyl
Protein binding capacity per mL of polymer	1.9 mg/mL 5.5 mg/mL 1.0 mg/mL 0.5 mg/mL Angiotensin Insulin Lysozyme Lysozyme
Bed height	50 mm 46 mm 44 mm 40 mm
Column volume	0.4 µL 1.6 µL 9.8 µL 0.76 mL 0.73 mL 0.65 mL
pH Range	2.5–8 1–14 1–14 1–14
Recommended flow rate	0.8–1.0 µL/min 2.5–3.0 µL/min 15–20 µL/min 0.5–4 mL/min 1–10 mL/min 1–16 mL/min
Maximum flow rate	1.2 µL/min 5.0 µL/min 30 µL/min 5 mL/min 14 mL/min 20 mL/min
Maximum pressure	5000 psi 2800 psi 2800 psi 2800 psi 34.4 Mpa 19.3 Mpa 19.3 Mpa 19.3 Mpa
Operating pressure	<2900 psi <1500 psi <1500 psi <1500 psi 20 Mpa 10.3 Mpa 10.3 Mpa 10.3 Mpa
Temperature	70 °C 70 °C 70 °C 70 °C
Solvent compatability	Most common organic solvents Most common organic solvents Most common organic solvents Most common organic solvents