|
| | | Micro Autosampler |
| The Micro Autosampler is an essential component of the Ultimate® 3000 HPLC Micro Systems and offers minimal gradient delay and low extra column volume that are crucial to high efficiency separations and short chromatographic run times. The Micro Autosampler uses an in-line, split-loop injection principle where the needle and sample loop are integral parts of the high-pressure fluidic path, which ensures 100% transfer of the sample volume and virtually eliminates carryover between sample injections. The Ultimate 3000 Micro Autosampler is strongly recommended for high throughput and fast LC and LC-MS. - The UltiMate Micro Autosamplers have only 1.2 μL of extra column volume to minimize dispersion during injection
- Peak area precision is typically <0.15% RSD for 2 μL injections
- Combination of a high-precision drive mechanism and a low gradient delay volume of 40 μL routinely delivers injector linearity values of r2=0.9990 from 1–20 μL
- Volumetric accuracy better than +0.5% for 20-μL injections guarantees consistent results
- Supports an injection volume range from 0.2 to 20 μL in the standard or thermostatted version
- Well plate compatible sample trays paired with low injection cycle times (<15 s for 5 μL) support high throughput LC and LC-MS applications
Performance Examples
| | | Micro Autosampler Specifications | | Sample Capacity | Dependent on sample trays:
15 x 10-mL
66 x 4-mL
120 x 1.8 mL
3 x 96 normal and deep well plates
3 x 384 deep well plates | | Injection Methods | In-line split-loop | | Injection Volume | 0.2–20 µL (settable: 0.01–25 µL) | | Injection Volume Accuracy | ±0.5% at 20 µL | | Injection Volume Precision | <0.3% RSD at 2 µL (typically <0.15% RSD) | | Linearity | Corr. coeff. >0.9999, RSD <0.5% at 1–20 µL | | Needle Wash | Active external needle wash | | Carryover | <0.005% for caffeine with external wash | | Injection cycle time | <15 s for 5 µL | | Optional Sample Thermostatting | 4–45°C, at least 22 °C below ambient | | Sample Temperature Accuracy | ±2 °C | | Dimensions (h x w x d) | 40 x 42 x 51 cm (16 x 16.5 x 20 in.) | | PC Connection | USB; USB hub with 3 sockets integrated | | Additional Specifications | Please see data sheet |
| | Order Information | | WPS-3000SL Micro UltiMate 3000 Micro Split-Loop Autosampler | 5822.0015 | | WPS-3000TSL Micro UltiMate 3000 Thermostatted Micro Split-Loop Autosampler | 5822.0025 |
| |
|
|  |  | Injectors and Autosamplers | | UltiMate® 3000 Injector and Autosampler Series delivers new levels of performance, reliability, and ease of use. |
| Modules | | | UltiMate ® 3000 LC modules achieve new levels of flexibility, performance, reliability, and ease of use. |
| LC and LC/MS Overview | Discover the UltiMate ® 3000 LCi series, and learn how LCi solutions can improve performance and productivity in your laboratory. |
| LCi Solutions |  | Find out how the UltiMate® 3000 LCi Series can provide you with LC solutions for your chromatographic challenges. |
|
|
