Dionex offers four polymer-based columns for the separation of proteins by ion exchange chromatography. Each of these ultra-high efficiency columns provides sufficient resolution to allow separation of protein isoforms that, in many cases, differ by only a single amino acid residue.

The ProPac^® HIC-10 is a new silica-based HPLC column for the separation of proteins by hydrophobic interaction chromatography. These HIC columns have been developed with multifunctional ligand attachments to provide enhanced hydrolytic stability under standard HIC elution conditions. The ProPac HIC-10 columns offer exceptional resolution and provide high loading capacity, making them ideal for protein separation and purification. 

The ProPac IMAC-10 Column is an HPLC column for the separation, by immobilized metal affinity chromatography (IMAC), of certain proteins and peptides with an affinity for a specific group of metals. This column represents the latest advancement in IMAC technology; it is a true high pressure, high resolution column capable of separating not only metal-binding proteins from non metal binding proteins, but in many cases also providing resolution of retained metal-binding proteins from one another.

ProSwift™ Monolith HPLC columns offer a unique monolith morphology that is ideal for fast, high-resolution separations of proteins. The columns feature low backpressure, high loading capacity, and stability over a wide pH range.  

The Acclaim^® 300 columns are a wide-pore line of C18 reversed-phase columns manufactured by Dionex for the separation of proteins, peptides, and other biological macromolecules. This line of columns extends the same unique bonding chemistry developed for the Acclaim 120 columns to a wide-pore silica substrate. The Acclaim 300 columns consist of 3-µ-diameter silica particles with 300-Å diameter pores, bonded with a highly characterized C18 phase. The unique bonding chemistry results in a high-density, highly uniform phase coverage with extensive end-capping.

Acclaim^® PepMap™ is a silica-based C18 stationary phase with virtually zero silanophilic activity, offering outstanding separation of peptides with minimal band broadening. As a result, TFA is no longer required in peptide mapping, allowing the use of solvent additives, such as formic acid, with substantially lower signal suppression and higher sensitivity in mass spectrometry (MS). Microcolumns packed with Acclaim PepMap are available from 75-µm i.d. up to 1.0-mm i.d. in different lengths and configurations to permit easy connection to MS.

Micro and Nano Precolumns are used for preconcentration of dilute samples, in sample cleanup, or in various column-switching techniques. Dilute samples can be effectively concentrated on the micro or nano precolumn by selecting the proper packing material. Micro and nano precolumns are used for fast sample concentration in combination with large-volume injections. Column lengths of 1 mm for nano precolumns (300 µm i.d.) and of 5 or 15 mm for micro precolumns (i.d. 300, 500, 800 µm, or 1.0 mm) shorten loading time, which results in reduced overall analysis time. Desalting and multidimensional (2-D, 3-D, etc.) chromatography are other applications.

Nano Precolumns are short columns (1 cm or 2 cm), with an inner diameter of 100 or 200 µm. They are used for preconcentration (focusing) of diluted samples, or in various column-switching techniques.

PepSwift ^TM  Monolithic columns for proteomics applications offer various benefits over particulate columns. The polymeric rod with porous structure, and no intra-particular void volumes, improves both mass transfer and separation efficiency, allowing for fast, high-resolution separation of proteins and peptides.

Dionex offers two approaches to amino acid analysis: (1) anion-exchange separation by AAA-Direct ^TM in which amino acids are detected directly, without the need for derivatization, or (2) anion-exchange separation with postcolumn derivatization. The AminoPac^® PA10 is the recommended column for AAA-Direct, whereas the AminoPac PA1 is used with postcolumn derivatization.

The DNAPac^® series of columns are polymer-based, anion-exchange columns that set the standard for oligonucleotide purity checks, fast screenings, and purification. The DNAPac PA200 offers exceptional resolution, phase stability over a broad pH range, and the ability to perform high-throughput separations. The DNAPac PA100 columns combine high-resolution separations with scalability for DNA analyses. Specially designed protocols using the Dionex BioLC^® system enable resolution on these columns of normal-length oligonucleotides (8- to 30-mer), extended-length oligonucleotides (30- to 70-mer), and with the DNAPac PA100, linear double-stranded DNA and supercoiled versus nicked/linear DNA.

Whether your interest is oligosaccharide mapping, mono- and disaccharide analysis, or monitoring sugars and sugar alcohols, a CarboPac ^TM high-performance anion-exchange (HPAE) column can help. The CarboPac family of columns has five columns to choose from, and each is optimized for a different class of compounds. Combined with pulsed amperometric detection (PAD)—which provides a means to detect the carbohydrates without derivatization—the CarboPac columns provide you with a simple, reliable technique to separate your sugars.

Dionex offers three trap columns for bio applications to reduce sample preparation and ensure your successful chromatographic separation. The BorateTrap^TM removes borate from the eluent and is recommended for sugar alcohol and monosaccharide applications. The AminoTrap^TM retains amino acids contaminants in carbohydrate separations.  The SugarTrap removes carbohydrate contaminants from amino acid separations.