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| ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms | |
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The ProPac® SCX-10 is a nonporous, strong, cation-exchange column for the separation closely related protein variants. The ProPac SCX-10 is a strong cation exchanger with sulfonate functional groups attached to a nonporous core. The unique nature
of the ProPac design imparts these columns with the ability to resolve protein variants with resolution unmatched by any other
vendor. Traditionally, porous resins have been used for column packing materials because of the increased surface area provided
by the pores. However, proteins, like other large molecules, are often too big to fit through these pores, which can result
in significant deterioration in peak shape through mass transfer restrictions. The ProPac resins are based on a nonporous
polymeric particle coated with a proprietary hydrophilic layer and functionalized with grafted linear ion-exchange moieties.
The nonporous core particle results in high-efficiency separations, the hydrophilic layer prevents unwanted secondary interactions
that can destroy a separation, and the grafted ion-exchange chains provide the ProPac columns with much higher capacity than
typical nonporous particles. The choice of using a strong or weak anion or cation exchanger is somewhat empirical, and is
based on the pI of the protein in question. ProPac SCX-10 columns are manufactured and tested under the strictest of specifications, resulting in unmatched column-to-column
and lot-to-lot reproducibility. Dionex offers these columns as a lot select package; three columns from one lot or three columns
each from a different lot, to assist the quality control scientist in the validation of their methods. The power of the ProPac
SCX-10 has been demonstrated in applications ranging from the resolution of glycosylated hemoglobins to hemoglobin sequence
variants. |
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