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| CarboPac PA1 Column for Routine Carbohydrate Analysis | |
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The CarboPacTM PA1 is the workhorse of all carbohydrate columns. Rugged, reliable, and reproducible, this column is used for the analysis
of monosaccharides, disaccharides, and for linear polysaccharide profiling. The CarboPac PA1 column is a general-purpose, anion-exchange column for the separation of mono-, disaccharides, and specific
oligosaccharides, with a simple isocratic eluent. Sialic acid analysis has also been demonstrated on this column. Detection
is direct using pulsed amperometric detection; no derivatization is required. Sensitivity on the CarboPac PA1 is not as high
as with the CarboPac PA20 or CarboPac PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis
of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods, including ISO/DIS
11292 for coffee authenticity, International Committee of Uniform Methods of Sugar Analysis (ICUMSA) for sugars in molasses
and AOAC Methods 996.04 and 2000.11. The CarboPac PA1 is the column of choice for high-resolution gradient separations of linear polysaccharides, such as maltodextrins
used for tableting pharmaceuticals, or those used as high-calorie nutritive supplements. The unique MicroBeadTM pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The
pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration. The performance of each column is verified with a mixture of three sugars to ensure reliable performance for the separation
of simple carbohydrates. Semipreparative chromatography is easy with the 9 × 250 mm CarboPac PA1 column. This column has identical
selectivity and efficiency with five times the capacity of the 4 × 250 mm CarboPac PA1 analytical column. |
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