The DNAPac® PA100 is a high-resolution, anion-exchange column that provides single-base resolution, is stable under denaturing conditions, is rugged and reliable, and can be readily scaled up. Capable of n, n – 1 resolution for oligonucleotides. Resolves oligonucleotides with secondary structures. Compatible with solvent, high pH, and high temperatures. Analyzes phosphorothioate-based clinical samples. Provides easy scale-up.
In recent years, a dramatic increase has occurred in the synthesis of nucleic acid oligomers, particularly as PCR primers. As a result, the need for high-speed purification and analysis of nucleic acids has grown. The MicroBeadTM resin technology used in the DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric nature of the DNAPac PA100 allows great flexibility in selecting the ideal conditions for your separation, including high pH, high temperature, and chaotropic agents. The DNAPac PA100 is packed with a pellicular anion-exchange resin comprised of a 13-µm-diameter nonporous polymeric substrate to which is bound quaternary amine-functionalized MicroBeads. The rapid mass-transport characteristics of this resin result in higher-resolution oligonucleotide separations than are possible with traditional macroporous resins or reversed-phase columns. Several unique features are incorporated into the packing material to provide unit-base resolution of synthetic oligonucleotides to 60 bases and beyond. Using the DNAPac PA100, synthetic oligonucleotides can be screened for production yield and failure sequences on a routine basis. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences of poly-G stretches.
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| DNAPac PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides Specifications | | Temperature range | Recommended operating temperature: N/A | | Typical operating conditions | 2400 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min | | Mobile phase compatibility | pH 2–12.5; 100% compatible with common organic solvents. Ionic form eluents: chloride, acetate, or bromide in lithium, sodium, or ammonium forms. | | Resin composition | 13-µm-diameter nonporous substrate (55% cross-linking) agglomerated with a 100-nm MicroBead alkyl quaternary ammonium functionalized latex (5% cross-linked). | | Anion-exchange capacity | ~40 OD260 (4 × 250 mm analytical column) | | Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. | | Dimensions | DNAPac PA100 Analytical Column:
2 × 250 mm DNAPac PA100 Guard Column:
2 × 50 mm DNAPac PA100 Analytical Column:
4 × 250 mm DNAPac PA100 Guard Column:
4 × 50 mm DNAPac PA100 Semipreparative Column:
9 × 250 mm DNAPac PA100 Semipreparative Column:
22 × 250 mm DNAPac PA100 Semipreparative Guard Column:
22 × 50 mm | | Maximum operating pressure | 4000 psi (27.9 MPa) |
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| Order Information | | DNAPac PA-100 Analytical Column (4 x 250 mm) | 043010 | | DNAPac PA-100 Semi-Preparative Column (9 x 250 mm) | 043011 | | DNAPac PA-100 Guard Column (4 x 50 mm) | 043018 | | DNAPac PA-100 Semi-Preparative Column (22 x 250 mm) | SP2091 | | FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 600 | SP3229 | | DNA Pac PA-100 Analytical Column (2 x 250 mm) | SP3686 | | FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 150 | SP4008 | | DNAPac PA-100 Guard Column (2 x 50 mm) | SP4016 | | DNAPac PA-100 Column (22 x 50 mm) | SP4513 | | FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 10 | SP4757 |
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