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| | | ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms |  | The ProPac® SCX-10 is a nonporous, strong, cation-exchange column for the separation closely related protein variants. Ion-exchange columns that provide characterization and quality control assessment of closely related protein variants Exceptionally high-resolution separations High-efficiency separations with excellent sample recovery Availability of different ProPac columns for flexibility in maximizing differences between closely related species
The ProPac SCX-10 is a strong cation exchanger with sulfonate functional groups attached to a nonporous core. The unique nature of the ProPac design imparts these columns with the ability to resolve protein variants with resolution unmatched by any other vendor. Traditionally, porous resins have been used for column packing materials because of the increased surface area provided by the pores. However, proteins, like other large molecules, are often too big to fit through these pores, which can result in significant deterioration in peak shape through mass transfer restrictions. The ProPac resins are based on a nonporous polymeric particle coated with a proprietary hydrophilic layer and functionalized with grafted linear ion-exchange moieties. The nonporous core particle results in high-efficiency separations, the hydrophilic layer prevents unwanted secondary interactions that can destroy a separation, and the grafted ion-exchange chains provide the ProPac columns with much higher capacity than typical nonporous particles. The choice of using a strong or weak anion or cation exchanger is somewhat empirical, and is based on the pI of the protein in question. ProPac SCX-10 columns are manufactured and tested under the strictest of specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex offers these columns as a lot select package; three columns from one lot or three columns each from a different lot, to assist the quality control scientist in the validation of their methods. The power of the ProPac SCX-10 has been demonstrated in applications ranging from the resolution of glycosylated hemoglobins to hemoglobin sequence variants.
| | ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications | | Maximum operating pressure |
3000 psi (21 MPa)
| | Maximum operating temperature | 60 °C Recommended operating temperature: <40 °C | | Typical operating conditions | UV detection at 1 mL/min Recommended flow rate: 1 mL/min | | Mobile phase compatibility |
pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and zwitterionic detergents. Incompatible with cationic detergents.
| | Resin composition | 10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the sulfonate functional groups. | | Anion-exchange capacity |
>100 mg protein on-column, depending upon the protein (4 × 250 mm analytical column)
| | Column construction |
PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.
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| | Order Information | | ProPac SCX-10 Analytical Column (4 x 250 mm) | 054995 | | ProPac SCX-10G Guard Column (4 x 50 mm) | 054996 | | ProPac SCX-10 Analytical Column (2 x 250 mm) | 063456 | | ProPac SCX-10G Guard Column (2 x 50 mm) | 063462 | | ProPac SCX-10 Semi-Preparative Column (9 x 250 mm) | 063700 | | ProPac SCX-10 Semi-Preparative Column (22 x 250 mm) | SP5522 | | ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin) | SP5727 | | ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin) | SP5728 |
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|  |  | ProPac SAX-10 Protein Separation Column |  | The ProPac® SAX-10 column is one of a unique family of ion-exchange columns capable of resolving protein isoforms and is particularly well suited to the analysis of dairy proteins. |
| ProPac WCX-10 Protein Analysis Column | | The ProPac® WCX-10 column one of a unique family of ion-exchange columns capable of resolving protein isoforms and is particularly well suited to the analysis of monoclonal antibodies. |
| ProPac PA1 Protein Column | | The ProPac® PA1 column is a strong anion-exchange column for the analysis and purification of hydrophilic anionic proteins and peptides. |
| ProPac HIC-10 Columns | | The ProPac HIC-10 column is a high capacity hydrophobic interaction chromatography column for the separation of proteins under non-denaturing conditions. |
| ProPac IMAC-10 Column |  | The ProPac IMAC-10 column is an HPLC IMAC column for the high resolution separation of certain metal-binding proteins. |
| ProSwift Monolith Columns |  | ProSwift™ Monolith HPLC columns offer a unique monolith morphology that is ideal for fast, high-resolution separations of proteins. The columns feature low backpressure, high loading capacity, and stability over a wide pH range. |
| Acclaim 300 Reversed-Phase Columns |  | The Acclaim® 300 columns are a wide-pore line of C18 reversed-phase columns manufactured by for the high-resolution separation of proteins, peptides, and other biological macromolecules. |
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