The ProPac® SAX-10 is a nonporous, strong cation-exchange column for the separation of closely related protein variants. Extremely high-resolution columns Higher capacity than standard nonporous particles High-efficiency peaks with excellent recovery Column variety that provides flexibility in maximizing differences of closely related species
The ProPac SAX-10 column is a strong anion exchanger with quaternary ammonium functional groups attached to a nonporous core. Traditionally, porous resins have been used for column packing materials because of the increased surface area provided by the pores. However, proteins, like other large molecules, are often too big to fit through these pores, which can result in significant peak shape deterioration through mass transfer restrictions. The ProPac resins are based on a nonporous polymeric particle coated with a proprietary hydrophilic layer and are functionalized with grafted, linear, ion-exchange moieties. The nonporous core particle provides the high-efficiency separations, the hydrophilic layer prevents unwanted secondary interactions that can destroy a separation, and the grafted ion-exchange chains provide much higher capacity than is typical with nonporous resins. Differences in selectivity between the ProPac WAX-10 and ProPac SAX-10 provide flexibility in maximizing resolution of closely related species. The choice to use a strong or weak anion or cation exchanger is somewhat empirical, and is based on the pI of the protein in question. As with all our ProPac columns, ProPac SAX-10 columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex offers these columns as a lot select package; three columns from one lot or three columns each from a different lot, to assist the quality control scientist in the validation of their methods. The power of the ProPac SAX-10 has been demonstrated in applications, including the resolution of phosphorylation variants (which is important in the characterization of biomacromolecules), sialylation variants that can be important in clinical diagnostics, and those used in the dairy industry to assess adulteration and the effects of processing.
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