The CarboPacTM MA1 column is a high-capacity, strong, anion-exchange column designed to deliver sensitive, rugged, and reliable separations of reduced sugars, without the need for derivatization. Recommended for the separation of reduced mono- and disaccharides No derivatization needed Separations effected using a simple hydroxide eluent Alditols and aldoses separated from one another in a single run
The CarboPac MA1 column consists of a macroporous polymeric resin to which is agglomerated the MicroBeadTM resin that contains the anion-exchange functional groups. The combination of a macroporous resin with the MicroBeads provides considerably greater capacity than other CarboPac columns. This greater capacity permits the use of higher sodium hydroxide concentrations in the eluents, which leads to greater ionization of sugar alcohols and thus better retention on the column. Analytes are eluted from the column at 0.4 mL/min using a simple sodium hydroxide eluent, either isocratically or with gradient elution. Specifically designed for reduced mono- and disaccharide alditol analyses, the CarboPac MA1 is also well suited for analysis of exoglycosidase-released neutral monosaccharides. Monosaccharides are moved away from the column void, enabling immediate evaluation of contaminating exoglycosidase activities. The CarboPac MA1 can provide baseline resolution of fucose, N-acetyl-(D)-glucosamine, N-acetyl-galactosamine, mannose, glucose, and galactose, as well as neutral oligosaccharides in the same separation.
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