The Acclaim^® PolarAdvantage (PA), is a polar-embedded column, that provides enhanced hydrolytic stability, where a single column can resolve both polar and nonpolar analytes. The Acclaim PA not only provides you with the flexibility to analyze both polar and nonpolar analytes on a single column, but also gives you the selectivity you expect.

• Single-run analysis of polar and nonpolar analytes
• Rugged column with symmetrical peak shapes
• Compatible with 100% aqueous mobile phases
• High reversed-phase capacity
• LC/MS compatible

Acclaim PA columns feature a polar-enhanced stationary phase. This phase consists of a C16 functional group bonded to the surface of ultrapure silica using a sulfonamide group coupled to an ether linkage. The unique chemistry of this polar-embedded phase provides excellent peak shapes for both basic and acidic compounds with a polarity, hydrophobicity, and selectivity similar to conventional C18 phases. The advantage is that you can transfer your C18 method over to the Acclaim PA and obtain a similar chromatography profile, but with better peak shapes and better column stability.

The Acclaim PA columns are compatible with 100% aqueous mobile phases, overcoming many of the limitations of conventional C8 and C18 reversed-phase columns. Because of their high solubility in water, many polar analytes are retained on reversed-phase columns only when the organic content of the eluent is very low. Conventional reversed-phase columns are unstable under these conditions and rapidly lose their ability to retain analytes. The patent-pending technology of the Acclaim PA column incorporates a region of hydrophilic functional groups between the hydrophobic C16 chain and the silica surface. This technology allows the surface to remain wetted, even in 100% aqueous mobile phases.

Another feature of the Acclaim PA columns is their stability under low-pH conditions. Although it is common to analyze polar analytes under low-pH conditions to reduce tailing of amine-containing compounds, under these conditions the lifetime of most conventional and many polar-embedded phases is greatly reduced due to cleavage of the bonded phase. The proprietary bonding of the Acclaim PA column resists hydrolytic attack by protecting the bonded phase at low pH. Because mass spectrometry (MS) applications typically add modifiers such as TFA or acetic acid to the mobile phase, the stability of the Acclaim PA at low pH makes it an ideal choice for LC/MS applications.

To meet the exacting needs of our customers, each Acclaim column is manufactured to stringent specifications to ensure column-to-column reproducibility. Each column is shipped with a lot validation sheet showing the test results and specifications for the lot of bonded silica packed into the column and an individual test chromatogram validating performance. The lot validation includes a chromatography-based metals test and tests for polar selectivity, hydrophobic steric selectivity, and base asymmetry. Production columns are individually tested for capacity and efficiency, and closely monitored for metals contamination. Reproducible column-to-column manufacturing, the use of ultrapure silica, and performance validation of every column prior to shipping, all ensure that the quality of your separations will be maintained month to month and year to year.