Ultrafast baseline separation of 21 Amino acids using the Acclaim RSLC 2 µm C18 (2.1 x 100 mm) column on the UltiMate 3000 RSLC system.

New Acclaim^® RSLC 2 µm Columns

Acclaim Rapid Separation Liquid Chromatography (RSLC) 2 µm columns achieve high-throughput separation without sacrificing chromatographic performance. These columns, together with the UltiMate^® RSLC system, provide ultrafast, high efficiency LC separations. The Acclaim RSLC columns are based on high-purity spherical porous silica gel. Currently, two column chemistries are available - Acclaim RSLC 2 µm C18 and Acclaim RSLC 2 µm PolarAdvantage II (PA2). Acclaim RSLC C18 2 µm material features high-density monomeric C18 chemistry with exhaustive end-capping. As a result, it provides the same selectivity of standard C18 phase and extremely low silanol activity. Acclaim RSLC PA2 2 µm materials features amide-embedded chemistry with excellent hydrolytic stability (pH 1.5 to 10). In addition, it provides selectivity complementary to its C18 counterpart.

Acclaim RSLC 3 µm Columns

Acclaim RSLC 3 µm columns achieve separation performance while maintaining low column pressure. A column inner diameter of 3 mm makes RSLC columns fully compatible with standard analytical LC instrumentation, and saves up to 85% solvent compared to a 4.6-mm column of otherwise identical features. Three packing phases are available for the Acclaim RSLC 3 µm columns: Acclaim 120 C18, Acclaim PolarAdvantage (PA), and Acclaim PolarAdvantage II (PA2).

RSLC that Is Easy to Use

• Accelerate separations up to fivefold using conventional LC equipment.

• Accelerate throughput up to 30-fold compared to conventional LC, when used in combination with UltiMate RSLC systems.

• Save 70 to 92% solvent with Acclaim RSLC columns.

• Require only 15 to 40% of sample volume traditionally required for LC separations.

• Ease of operation (low back-pressure and more resistance to column clogging) ensures simple, fast, and reliable separations.

Intelligent Solutions for RSLC

The Dionex complete solution for RSLC combines Acclaim column technology with UltiMate 3000 RSLC systems. The 2 µm columns are packed with both C18 reversed-phase packing and PA2 (amide-polar embedded) packing; in both 2.1 x 100 mm and 2.1 x 50 mm formats. The 3 µm columns are available in 3 mm ID and 33 mm or 75 mm lengths, packed with 3 µm stationary phases. Acclaim RSLC columns integrate with Dionex UltiMate 3000 RSLC systems to provide superior performance, reliability, and ease of use.

For Acclaim RSLC 2 µm and 3 µm column ordering information, contact your local sales representative.