BioLC Applications

Configure the right biochromatography system for your application.  Whether you are analyzing carbohydrates, amino acids, proteins, peptides, or nucleic acids, biologically active small molecules, Dionex offers a system that fits your needs.

Carbohydrates   

Couple high-performance anion chromatography with pulsed amperometric detection for direct quantification of:

• Simple, direct determination of monosaccharides (such as glucose, fructose, and mannose), disaccharides (such as sucrose and maltose), trisaccharides, alditols (such as sorbitol and mannitol), glycols (such as glycerols and propylene glycol), and other carbohydrates
• Sensitivities in the picomole to femtomole range
• Quantitative determination of glycoprotein monosaccharides
• Single-unit resolution of glycoprotein oligosaccharides
• Glycoprotein mono- and oligosaccharides
• Sialic acids
• Carbohydrates and glycols in fermentation broths, foods, and more
• Polysaccharides

Amino Acids 

Using integrated pulsed amperometric detection (IPAD), both primary and secondary amino acids can be sensitively determined—with no derivatization required.

• Amino acids and sugars in:
  • Fermentation broths
  • Cell cultures
  • Physiological fluids
  • Foods and beverages
• Profiling of amino acids, carbohydrates, and amino sugars in protein hydrolysates
• Detection limits in the low- to sub-picomole range, without derivatization
• Approximately 50 times more sensitive than ninhydrin-based analyzers and competitive with precolumn derivatization techniques
• Determine amino acids, phosphoamino acids, carbohydrates, amino sugars, and phosphoamino acids—in the same run
• Easy quantification of amino acids such as tryptophan and sulfur-containing amino acids

Proteins and Peptides 

Achieve high-resolution separations for:

• Peptide mapping
• Monitoring protein variant heterogeneity due to:
  • Sialylation
  • C-terminal truncation
  • N-terminal pyroglutamate formation
  • Asparagines deamidation
• Single base residue differences in normal and extended (8–70 mer) oligonucleotides
• Supercoiled plasmid and linear DNA separation
• PCR products

Nucleic Acids

• n – 1 separations of synthetic oligonucleotides greater than 60 bases
• Separation of phosphorothioate oligonucleotides
• Separation of proteins from their deamidated or phosphorylated forms